Stable shuttle vectors for Neisseria gonorrhoeae, Haemophilus spp. and other bacteria based on a single origin of replication
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- 作者：Pagotto ; F.J. ; Salimnia ; H. ; et. al.
- 关键词：Cloning vector ; Complementation ; GFP ; Gonococcus ; Plasmid ; cat ; gene encoding chloramphenicol acetyl-transferase ; GCMB ; GC-agar base ; GCMBK ; GCMB supplemented with Kellogg's supplement ; GFP ; green fluorescent protein ; Inc ; incompatibility ; kb ; kilobase pa
- 刊名：Gene
- 出版年：2000
- 出版时间：February 22, 2000
- 年：2000
- 卷：244
- 期：1-2
- 页码：13-19
- 全文大小：582 K
文摘
An origin of replication (ori) was obtained from a naturally occurring β-lactamase-producing plasmid isolated from Neisseria gonorrhoeae and used to construct shuttle vectors capable of replicating in N. gonorrhoeae, Haemophilus ducreyi, Haemophilus influenzae and Escherichia coli. Using the gonococcal proAB genes, we complemented proline-requiring N. gonorrhoeae F62 and E. coli HB101 in trans. The first demonstration of the expression of the green fluorescent protein (GFP) in either N. gonorrhoeae or H. ducreyi was shown using this vector, indicating that GFP may be a useful tool in the analysis of these organisms. This is the first report of a gonococcal vector based on a broad host range, genetically defined ori, and should facilitate the molecular analysis of gonococcal and Haemophilus genes.