A simple, rapid and cost-effective process for production of latex clearing protein to produce oligopolyisoprene molecules

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• 作者：R. Andler^a ; ^{rodrigo.andler.o@gmail.com} ; A. Steinbü ; chel^a ; ^b ; ^{steinbu@uni-muenster.de}
• 关键词：Latex clearing protein ; Auto induction medium ; Ammonium sulfate precipitation ; Rubber biodegradation ; Poly(cis-1 ; 4-isoprene)
• 刊名：Journal of Biotechnology
• 出版年：2017
• 出版时间：10 January 2017
• 年：2017
• 卷：241
• 期：Complete
• 页码：184-192
• 全文大小：1189 K
• 卷排序：241

文摘

The overexpression of the rubber-cleaving dioxygenase Lcp1VH2 in Escherichia coli using an auto induction medium was described A two steps salting out precipitation with ammonium sulfate was developed for the purification of Lcp1VH2. Oligo(cis-1,4-isoprene) molecules were obtained by using the partially purified Lcp1VH2.