p66^Shc gene has a pro-apoptotic role in human cell lines and it is activated by a p53-independent pathway

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• 作者：Luca Tiberi ; Amir Faisal ; Matteo Rossi ; Lucia Di Tella ; Claudio Franceschi and Stefano Salvioli
• 关键词：p66^Shc ; Oxidative stress ; Apoptosis ; Human cell lines ; Serine phosphorylation
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2006
• 出版时间：7 April 2006
• 年：2006
• 卷：342
• 期：2
• 页码：503-508
• 全文大小：196 K

文摘

p66^Shc protein has been proposed to be an indispensable factor for p53-dependent, mitochondria-mediated apoptosis in mice. Here, we show that p66^Shc plays a pro-apoptotic role also in cell lines of human origin such as SaOs-2 and HeLa, where p53 is either absent or inactivated, thus, suggesting that p66^Shc pro-apoptotic role is independent from the presence of a functional form of p53. The active form of p66^Shc is phosphorylated in Serine 36. We confirm the importance of Serine 36 phosphorylation for p66^Shc pro-apoptotic role, and our results suggest that the kinase involved in this process is activated independently from p53.