No effect of verapamil on the local bone response to in vivo mechanical loading

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• 作者：Samneg&aring ; rd ; Eva ; Cullen ; Diane M. ; Akhter ; Mohammed P. ; Kimmel ; Donald B.
• 刊名：Journal of Orthopaedic Research
• 出版年：2001
• 出版时间：March, 2001
• 年：2001
• 卷：19
• 期：2
• 页码：328-336
• 全文大小：180 K

文摘

Verapamil, a calcium channel blocker, alters the intracellular calcium concentration in bone cells in vitro, while mechanical loading stimulates calcium channels. The purpose of this study was to examine the effect of systemic verapamil treatment on the bone response to in vivo external mechanical loading. Female rats (age 5–6 months) were divided into six groups. Half were verapamil treated (0.75 mg/ml drinking water) for 12 weeks. After 8 weeks of treatment, the right tibia was loaded by a four-point bending device. In one set of verapamil and control groups, the right tibia was loaded at 31.8±0.2 N (36 cycles, 2 Hz, 3 d/wk) for four weeks. A second set was loaded at 40.1±0.3 N and the third set remained nonloaded. Tibial cortical bone formation and femur bone mineral density (BMD) were evaluated. With loading, bone formation was similarly elevated in loaded tibia of verapamil and control rats (P<0.003). However, periosteal bone formation (P<0.001) in the nonloaded tibia, and femoral diaphysis BMD (P<0.04) were greater in verapamil rats than in controls.