Molecular cloning and structural characterization of Ecdysis Triggering Hormone from Choristoneura fumiferana
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- 作者：Bhagath Kumar P.a ; Kasi Viswanath K.a ; Tuleshwori Devi S.a ; Sampath Kumar R.a ; Daniel Doucetb ; Arthur Retnakaranb ; Peter J. Krellc ; Qili Fengb ; Dinakara Rao Ampasalaa ; ampasaladr@bicpu.edu.in" class="auth_mail" title="E-mail the corresponding author
- 关键词：Cloning ; Developmental studies ; Ecdysis ; ETH ; In silico studies
- 刊名：International Journal of Biological Macromolecules
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：88
- 期：Complete
- 页码：213-221
- 全文大小：2220 K
文摘
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Full length cDNA of Choristoneura fumiferana ETH was cloned and expression profile was studied.
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The 3D structure of CfETH peptide was modelled and validated.
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Molecular dynamic simulations studies supported the stability of the peptide model.
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HTVS was performed for the modelled peptide taking a library of insecticides to identify hit compounds.
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Docking studies revealed that the compound-1 can be considered as a hit compound.