Polyethylene imine-based receptor immobilization for label free bioassays

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• 作者：S. Kurunczi^a ; ^{kurunczi.sandor@ttk.mta.hu}Author Vitae ; A. Hainard^bAuthor Vitae ; K. Juhasz^aAuthor Vitae ; D. Patko^aAuthor Vitae ; N. Orgovan^aAuthor Vitae ; N. Turck^bAuthor Vitae ; J.C. Sanchez^bAuthor Vitae ; R. Horvath^aAuthor Vitae
• 关键词：Room temperature immobilization ; Polyethylene imine (PEI) ; Label free sensor ; Neutravidin ; C-reactive protein (CRP) ; OWLS
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2013
• 出版时间：May, 2013
• 年：2013
• 卷：181
• 期：Complete
• 页码：71-76
• 全文大小：590 K

文摘

Polyethylene imine (PEI) based immobilization of antibodies is described and the concept is proved on the label free assay of C-Reactive Protein (CRP). This novel immobilization method is composed of a hyperbranched PEI layer which was deposited at a high pH (9.5) on the sensor surface. The free amino groups of PEI were derivatized with neutravidin by Biotin N-hydroxysuccinimide ester and the biotinylated anti-CRP antibody immobilized on this layer. Direct binding assay of recombinant CRP was successfully performed in the low ¦Ìg/ml concentrations using a label free optical waveguide biosensor.