Gelatin-based membrane containing usnic acid-loaded liposome improves dermal burn healing in a porcine model

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• 作者：Paula Santos Nunes^a ; ^{paulanunes_se@yahoo.com.br" class="auth_mail" title="E-mail the corresponding author} (Prof.) ; Alessandra Silva Rabelo^b ; Jamille Cristina Campos de Souza^b ; Bruno Vasconcelos Santana^b ; Thailson Monteiro Menezes da Silva^b ; Mairim Russo Serafini^b ; Paula dos Passos Menezes^b ; Bruno dos Santos Lima^b ; Juliana Cordeiro Cardoso^c ; Jú ; lio Cé ; sar Santana Alves^d ; Luiza Abrahã ; o Frank^e ; Sí ; lvia Stanisç ; uaski Guterres^e ; Adriana Raffin Pohlmann^e ; Malone Santos Pinheiro^c ; Ricardo Luiz Cavalcanti de Albuquerque Jú ; nior^c ; Adriano Antunes de Souza Araú ; jo^b
• 关键词：Burn ; Porcine ; Gelatin membrane ; Liposomes ; Usnic acid
• 刊名：International Journal of Pharmaceutics
• 出版年：2016
• 出版时间：20 November 2016
• 年：2016
• 卷：513
• 期：1-2
• 页码：473-482
• 全文大小：4298 K

文摘

There are a range of products available which claim to accelerate the healing of burns; these include topical agents, interactive dressings and biomembranes. The aim of this study was to assess the effect of a gelatin-based membrane containing usnic acid/liposomes on the healing of burns in comparison to silver sulfadiazine ointment and duoDerme^® dressing, as well as examining its quantification by high performance liquid chromatography. The quantification of the usnic acid/liposomes was examined using high performance liquid chromatography (HPLC) by performing separate in vitro studies of the efficiency of the biomembranes in terms of encapsulation, drug release and transdermal absorption. Then, second-degree 5 cm² burn wounds were created on the dorsum of nine male pigs, assigned into three groups (n = 3): SDZ – animals treated with silver sulfadiazine ointment; GDU – animals treated with duoDerme^®; UAL – animals treated with a gelatin-based membrane containing usnic acid/liposomes. These groups were treated for 8, 18 and 30 days. In the average rate of contraction, there was no difference among the groups (p> 0.05). The results of the quantification showed that biomembranes containing usnic acid/liposomes were controlled released systems capable of transdermal absorption by skin layers. A macroscopic assay did not observe any clinical signs of secondary infections. Microscopy after 8 days showed hydropic degeneration of the epithelium, with intense neutrophilic infiltration in all three groups. At 18 days, although epidermal neo-formation was only partial in all three groups, it was most incipient in the SDZ group. Granulation tissue was more exuberant and cellularized in the UAL and GDU groups. At 30 days, observed restricted granulation tissue in the region below the epithelium in the GDU and UAL groups was observed. In the analysis of collagen though picrosirius, the UAL group showed greater collagen density. Therefore, the UAL group displayed development and maturation of granulation tissue and scar repair that was comparable to that produced by duoDerme^®, and better than that produced by treatment with sulfadiazine silver ointment In addition, the UAL group showed increased collagen deposition compared to the other two groups.