Genotoxic effect of Bothrops snake venoms and isolated toxins on human lymphocyte DNA

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• 作者：Silvana Marcussi^a ; ^b ; ^{marcussi@dqi.ufla.br} ; Rodrigo G. Stá ; beli^c ; Norival A. Santos-Filho^a ; Danilo L. Menaldo^a ; Luciana L. Silva Pereira^b ; Juliana P. Zuliani^c ; Leonardo A. Calderon^c ; Saulo L. da Silva^d ; Lusâ ; nia M. Greggi Antunes^a ; Andreimar M. Soares^c ; ^{andreims@hotmail.com} ; ^{andreimar@fiocruz.br}
• 关键词：Genotoxic potential ; Micronucleus ; Comet ; Snake venom toxins ; Bothrops snake
• 刊名：Toxicon
• 出版年：2013
• 出版时间：April, 2013
• 年：2013
• 卷：65
• 期：Complete
• 页码：9-14
• 全文大小：177 K

文摘

In the present study, micronucleus with cytokinesis blocking and comet assays were used to evaluate the genotoxic potential of m>Bothrops jararacussum>, m>Bothrops atroxm>, m>Bothrops moojeni, Bothrops alternatusm> (m>Rhinocerophis alternatusm>) and m>Bothrops brazilim> snake venoms, and also of some isolated toxins (MjTX-I, BthTX-I and II myotoxins, BjussuMP-II metalloprotease, and BatxLAAO l-amino acid oxidase) on human lymphocytes. Significant DNA damages were observed, indicating genotoxic potential after exposure of the lymphocytes to the toxins BthTX-I, II and BatxLAAO compared to untreated and Cisplatin-treated controls, which were able to induce greater formation of micronuclei. m>B. brazilim>, m>B. jararacussum> and m>B. atroxm> crude venoms also presented genotoxic potential, and the latter two induced DNA breakage 5 times more often than in normal environmental conditions (control without treatment). m>B. jararacussum> venom and its isolated toxins, as well as an LAAO from m>B. atroxm>, were able to cause lymphocyte DNA breakage in the comet test with more than 85 % ?damage levels. The DNA damage evaluation allows a widening of the toxic-pharmacological characterization of snake venoms and their toxins and also contributes to the understanding of the mechanisms of action of these molecules in several human pathologies.