SAP97 promotes the stability of Na_x channels at the plasma membrane

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• 作者：Masahito Matsumoto^a ; Akihiro Fujikawa^a ; Ryoko Suzuki^a ; Hidetada Shimizu^a ; Kazuya Kuboyama^a ; Takeshi Y. Hiyama^a ; ^b ; Randy A. Hall^c ; Masaharu Noda^a ; ^b ; ^{madon@nibb.ac.jp}
• 关键词：Sodium channel ; PDZ array ; PDZ-binding motif ; MAGUK protein ; Subfornical organ ; Astrocyte
• 刊名：FEBS Letters
• 出版年：2012
• 出版时间：2 November, 2012
• 年：2012
• 卷：586
• 期：21
• 页码：3805-3812
• 全文大小：1202 K

文摘

Na_x is a sodium-level sensor for body fluids expressed in the circumventricular organs in the brain. Na_x has a putative PSD-95/Disc-large/ZO-1 (PDZ)-binding motif at the carboxyl (C)-terminus. Here we found that several PDZ proteins bind to Na_x by PDZ-array overlay assay. Among them, synapse-associated protein 97 (SAP97/DLG1) was coexpressed with Na_x in the subfornical organ. In C6 glioblastoma cells, destruction of the PDZ-binding motif of Na_x or depletion of SAP97 resulted in a decrease in cell-surface Na_x, which was attenuated with inhibitors of endocytosis. These results indicate that SAP97 contributes to the stabilization of Na_x channels at the plasma membrane.

Structured summary of protein interactions

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