Countercurrent chromatography separation of saponins by skeleton type from Ampelozizyphus amazonicus for off-line ultra-high-performance liquid chromatography/high resolution accurate mass spectrometry analysis and characterisation
Methodologies were developed for separation and characterisation of butanol extract from Ampelozizyphus amazonicus bark. CCC separation of saponins by skeleton type from butanol extract produced oleane type and dammarane type groups. The characterisation of saponins was done by UHPLC–MS. Unusual aglycones saponins as C30 and C31 keto-dammarane were characterized by UHPLC–MS.