DNA vaccine delivery by densely-packed and short microprojection arrays to skin protects against vaginal HSV-2 challenge

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• 作者：Angela Shaulov Kask ; Xianfeng Chen ; Joshua O. Marshak ; Lichun Dong ; Misty Saracino ; Dexiang Chen ; Courtney Jarrahian ; Mark A. Kendall ; David M. Koelle
• 关键词：DNA vaccine ; Microprojection ; Herpes simplex virus ; Skin
• 刊名：Vaccine
• 出版年：2010
• 出版时间：3 November 2010
• 年：2010
• 卷：28
• 期：47
• 页码：7483-7491
• 全文大小：291 K

文摘

There is an unmet medical need for a prophylactic vaccine against herpes simplex virus (HSV). DNA vaccines and cutaneous vaccination have been tried for many applications, but few reports combine this vaccine composition and administration route. We compared DNA administration using the Nanopatch™, a solid microprojection device coated with vaccine comprised of thousands of short (110 μm) densly-packed projections (70 μm spacing), to standard intramuscular DNA vaccination in a mouse model of vaginal HSV-2 infection. A dose-response relationship was established for immunogenicity and survival in both vaccination routes. Appropriate doses administered by Nanopatch™ were highly immunogenic and enabled mouse survival. Vaginal HSV-2 DNA copy number day 1 post challenge correlated with survival, indicating that vaccine-elicited acquired immune responses can act quickly and locally. Solid, short, densely-packed arrays of microprojections applied to the skin are thus a promising route of administration for DNA vaccines.