Optimization of aeration and agitation rates to improve cellulase-free xylanase production by thermotolerant Streptomyces sp. Ab106 and repeated fed-batch cultivation using agricultural waste

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• 作者：Charin  ; Techapun¹ ; Naiyatat  ; Poosaran¹ ; Masanori  ; Watanabe² ; Ken  ; Sasaki  ; ;   ; ² ;   ; ^&lowast ; &lowast ; ;   ; ^{sasaki@g.hkg.ac.jp}
• 关键词：aeration ; agitation ; cellulase-free xylanase ; fed-batch
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2003
• 出版时间：2003
• 年：2003
• 卷：95
• 期：3
• 页码：298-301
• 全文大小：378 K

文摘

Thermostable cellulase-free xylanase was produced by Streptomyces sp. Ab106 using agricultural waste, sugar cane bagasse, as the substrate at 50¡ãC and pH 7.0. The central composite faccentered experimental design was applied to evaluate the optimal agitation and aeration rates in a 5-l fermentor. The highest activity (16.0¡À0.5 IU/ml) was obtained at an aeration rate of 1 vvm and an agitation rate of 150 rpm (k_L a = 351 h^?). Using the repeated fed-batch cultivation technique, the maximum xylanase activity of 32¡À1 IU/ml was obtained during the second cycle of repeated fed-batch culture.