Resolution of Specific Nucleotide Mismatches by Wild-Type and AZT-Resistant Reverse Transcriptases during HIV-1 Replication
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- 作者：Siarhei Kharytonchyk ; Steven R. King ; Clement B. Ndongmo1 ; Krista L. Stilger ; Wenfeng An2 ; Alice Telesnitsky ; ateles@umich.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：RT ; reverse transcriptase ; WT ; wild-type ; AZTR ; AZT-resistant ; RIO ; retroviral inside-out ; LTR ; long terminal repeat ; ppt ; polypurine tract ; RSV ; Rous sarcoma virus ; PBS ; primer binding site ; RRE ; Rev response element
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：5 June 2016
- 年：2016
- 卷：428
- 期：11
- 页码：2275-2288
- 全文大小：827 K
文摘
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The dissection of HIV-1 reverse transcriptase intracellular error mechanisms is challenging.
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A novel system to study mismatch resolution during HIV-1 replication was developed.
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Outcomes for wild-type and an AZT-resistant reverse transcriptase were quantified using three approaches.
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Most mismatches were extended to similar high extents by both enzymes.
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Striking mechanistic differences were revealed during dC–rC mispair resolution.