Revealing calcium fluxes by analyzing inhibition dynamics in action potential clamp

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• 作者：Martin Laasmaa ; Rikke Birkedal ; ^{rikke@sysbio.ioc.ee} ; Marko Vendelin ; ^{markov@ioc.ee}
• 关键词：AP ; action potential ; CICR ; Ca2+-induced Ca2+-release ; ECC ; excitation-contraction coupling ; LTCC ; L-type Ca2+ channel ; NCX ; Na+/Ca2+-exchange ; NCXrev ; Na+/Ca2+-exchange in reverse mode ; NIF ; nifedipine ; SERCA ; sarcoendoplasmic reticulum Ca2+-ATPase ; SR ; sarcoplasmic reticulum
• 刊名：Journal of Molecular and Cellular Cardiology
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：100
• 期：Complete
• 页码：93-108
• 全文大小：2698 K
• 卷排序：100

文摘

Novel method to quantify the Ca2+ fluxes involved in excitation contraction coupling The method is based on analysis of action potential clamp data by mathematical model. Method was tested against synthetic data and applied to reveal Ca2+ fluxes in trout. In trout, NCXrev is the main transmembrane Ca2+ influx pathway. In trout, 50% of Ca2+ transient is attributable to CICR from SR.