Algal oligosaccharides ameliorate osteoporosis via up-regulation of parathyroid hormone 1-84 and vascular endothelial growth factor

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• 作者：Wang Li^a ; Wang Haiya^a ; ^{haiyawsh@163.com" class="auth_mail" title="E-mail the corresponding author} ; Fang Ningyuan^a
• 关键词：Osteoporosis ; Bone density ; Sargassum ; Oligosaccharides ; Parathyroid hormone ; Vascular endothelial growth factor A ; MG-63 human osteoblastic cells
• 刊名：Journal of Traditional Chinese Medicine
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：36
• 期：3
• 页码：332-339
• 全文大小：998 K

文摘

To determine whether algal oligosaccharide affects the levels of parathyroid hormone 1-84 (PTH1-84) and vascular endothelial growth factor (VEGF).

Methods

spara140">An osteoporosis rat model was established via bilateral ovariectomy. The model rats were fed algal oligosaccharides (molecular weights: 600-1, 200 Da) for 4 months. Bone mineral density (BMD) was then measured. MG-63 human osteoblastic cells were treated with algal oligosaccharides. The expression of PTH1-84 and VEGF was then examined. Oligosaccharide-treated cells were transfected with PTH1-84 short hairpin RNA (shRNA), VEGF shRNA, and PTH1-84-VEGF small interfering RNA (siRNA). The growth rates were then compared between transfected and non-transfected cells.

Results

spara150">Algal oligosaccharides increased the BMD of the osteoporosis rat model compared with untreated controls (P < 0.05). When MG-63 cells were treated with algal oligosaccharides, the growth rate increased by 25% compared with the control group at day 3 (P < 0.05). In addition, the expression of PTH84 and VEGF was enhanced. Conversely, when the cells were transfected with PTH84 shRNA, VEGF shRNA, or PTH1-84-VEGF siRNA, the growth rate was decreased by 17%, 35% and 70%, respectively, compared with controls at day 3 (P < 0.05).

Conclusion

spara160">Algal oligosaccharides ameliorate osteoporosis via up-regulation of PTH1-84 and VEGF. Algal oligosaccharides should be developed as a potential drug for osteoporosis treatment.