Composition and anti-inflammatory effect of polysaccharides from Sargassum horneri in RAW264.7 macrophages

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• 作者：Zheng-Shun Wen^a ; ^{zswenmr@163.com" class="auth_mail" title="E-mail the corresponding author} ; Xing-Wei Xiang^b ; Huo-Xi Jin^a ; Xiang-Yang Guo^a ; Li-Jia Liu^a ; Yan-Na Huang^c ; Xiao-Kun OuYang^a ; You-Le Qu^a ; ^{youle1960@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Sargassum horneri polysaccharides ; Anti-inflammatory ; RAW264.7 macrophages
• 刊名：International Journal of Biological Macromolecules
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：88
• 期：Complete
• 页码：403-413
• 全文大小：1848 K

文摘

Sulfated polysaccharides extracted from brown marine algae have been shown to possess a variety of biological activities. We assessed the potential activity of the sulfated polysaccharide from Sargassum horneri (SP) and its isolated two major components (fraction-1 (F1) and fraction-2 (F2)), on anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. In the present study, analysis of polysaccharide chemical composition found that the constituent ratios of sulfate ester and fucose in SP and F1 were 4.95% vs 7.6%, and 4.48% vs 55.9%, respectively, suggesting that F1 may be a major sulfated polysaccharide containing fucose. Meanwhile, our findings demonstrated that TNF-α secretion levels were significantly (P < 0.05) decreased by SP and F1 treatments in LPS-stimulated RAW264.7 cells in a dose-dependent manner under the preventive and repair experimental models. Pro-/anti-inflammatory (TNF-α/IL-10) cytokines secretion ratios by LPS-stimulated RAW264.7 macrophages were significantly (P < 0.05) inhibited by SP and F1 treatments, particularly by F1 (at high dose, 200 μg/ml). Moreover, NO release and iNOS activity were significantly (P < 0.05) inhibited by F1. Collectively, the present study suggested that purified component, F1 from SP, had strong anti-inflammatory effects on LPS-stimulated RAW264.7 macrophages in the preventive and repair manner through inhibiting TNF-α secretion levels and NO release.