Detection of ionic liquid stable cellulase produced by the marine bacterium Pseudoalteromonas sp. isolated from brown alga Sargassum polycystum C. Agardh

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• 作者：Nitin Trivedi ; Vishal Gupta ; C.R.K. Reddy ; ^{crkcsmcri@gmail.com} ; ^{crk@csmcri.org} ; Bhavanath Jha
• 关键词：Cellulase ; Cellulose ; Energy ; Enzyme stability ; Ionic liquid
• 刊名：Bioresource Technology
• 出版年：2013
• 出版时间：March, 2013
• 年：2013
• 卷：132
• 期：Complete
• 页码：313-319
• 全文大小：686 K

文摘

An extracellular cellulase produced by marine bacterium Pseudoalteromonas sp. was studied for its activity and stability in six different ionic liquids (ILs) over a wide range of concentrations (1-20 % v/v) and compared with aqueous medium as control. Enzyme showed its optimal activity at 45 ¡ãC and at pH 5 in control. Although the activity varied with the type of IL and its concentration used, the activity measured at 5 % (v/v) was maximum with [EMIM]Br followed by [EMIM]Ac, [BMIM]Cl, [C₂MIM][CH₃SO₃], [BMIM][OTF] and [BMPL][OTF] with 115 % , 104.7 % , 102.2 % , 98.33 % , 93.84 % and 92.67 % , respectively, and >80 % activity at 15 % (v/v) in all ILs. The enzyme stability at 5 % (v/v) IL concentration for 36 h was superior to commercial cellulase. The cellulase activity enhanced by 1.35- to 1.72-fold over control when 5 % (v/v) IL based reaction medium with algal biomass was used and thus showed potentials for saccharification of biomass in a single step process.