Striking antigen recognition diversity in the Atlantic salmon T-cell receptor α/δ locus

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• 作者：Ryosuke Yazawa ; Glenn A. Cooper ; Peter Hunt ; Marianne Beetz-Sargent ; Adrienne Robb ; Melanie Conrad ; Linda McKinnel ; Stacy So ; Stuart Jantzen ; Ruth B. Phillips ; William S. Davidson ; Ben F. Koop
• 关键词：Atlantic salmon ; T-cell receptor α ; /δ ; locus ; Antigen recognition ; Gene rearrangement ; Adaptive immunity
• 刊名：Developmental and Comparative Immunology
• 出版年：2008
• 出版时间：2008
• 年：2008
• 卷：32
• 期：3
• 页码：204-212
• 全文大小：821 K

文摘

The complete TCR α/δ locus of Atlantic salmon (Salmo salar) has been characterized and annotated. In the 900 kb TCR α/δ locus, 292 Vα/δ segments and 123 Jα/δ segments were identified. Of these, 128 Vα/δ, 113 Jα, and a Jδ segment appeared to be functional as they lacked frame shifts or stop codons. This represents the largest repertoire of Vα/δ and Jα segments of any organism to date. The 128 functional Vα/δ segments could be grouped into 29 subgroups based upon 70 % nucleotide similarity. Expression data confirmed the usage of the diverse repertoire found at the genomic level. At least 99 Vα, 13 Vδ 86 Jα, 1 Jδ, and 2 Dδ segments were used in TCR α or δ transcription, and 652 unique genes were identified from a sample of 759 TCRα cDNA clones. Cumulatively, the genomic and expression data suggest that the Atlantic salmon T-cell receptor has enormous capacity to recognize a wide diversity of antigens.