Co-expression of human ABCG5 and ABCG8 in insect cells generates an androstan stimulated membrane ATPase activity

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• 作者：Marianna Mü ; ller ; Izabella Klein ; Szilá ; rd Kopá ; csi ; Alan T. Remaley ; É ; va Rajnavö ; lgyi ; Balá ; zs Sarkadi ; Andrá ; s Vá ; radi
• 关键词：Sitosterolemia ; Steroid hormones ; ATP binding cassette transporters
• 刊名：FEBS Letters
• 出版年：2006
• 出版时间：13 November 2006
• 年：2006
• 卷：580
• 期：26
• 页码：6139-6144
• 全文大小：589 K

文摘

Mutations in the ATP-binding cassette (ABC) proteins ABCG5 or ABCG8 cause sitosterolemia, a condition with increased accumulation of plant sterols. Upon high level expression of the ABCG5 and ABCG8 proteins in baculovirus-Sf9 cell expression system we found a distinct, vanadate sensitive ATPase activity in isolated membrane preparations only when the two proteins were co-expressed. This ATPase activity was significantly stimulated by the addition of certain androgen hormones and analogs, and was effectively inhibited by progesterone. Our results provide a new aspect of biochemical and functional characterization of the ABCG5/ABCG8 proteins and their possible involvement in steroid hormone transport or regulation.