Ins and outs of the ABCG2 multidrug transporter: An update on in vitro
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- 作者：Csilla Hegedű ; s ; Gergely Szaká ; cs ; Lá ; szló ; Homolya ; Tamá ; s I. Orbá ; n ; Á ; gnes Telbisz ; Má ; rton Jani ; Balá ; zs Sarkadi
- 关键词：ABCG2 ; Multidrug resistance ; ADME-Tox ; Membrane-based assays ; Cellular assays ; Conformation sensitive antibody ; Fluorescent proteins
- 刊名：Advanced Drug Delivery Reviews
- 出版年：2009
- 出版时间：31 January 2009
- 年：2009
- 卷：61
- 期：1
- 页码：47-56
- 全文大小：348 K
文摘
The major aim of this chapter is to provide a critical overview of the in vitro methods available for studying the function of the ABCG2 multidrug transporter protein. When describing the most applicable assay systems, in each case we present a short overview relevant to ABC multidrug transporters in general, and then we concentrate on the tools applicable to analysis of substrate-drug interactions, the effects of potential activators and inhibitors, and the role of polymorphisms of the ABCG2 transporter. Throughout this chapter we focus on recently developed assay systems, which may provide new possibilities for analyzing the pharmacological aspects of this medically important protein.