One-step labeling of degenerative neurons in unfixed brain tissue samples using Fluoro-Jade C

详细信息    查看全文

• 作者：Qiang Gu ; ^{qiang.gu@fda.hhs.gov} ; Larry C. Schmued ; Sumit Sarkar ; Merle G. Paule ; Bryan Raymick
• 关键词：Neurodegeneration ; Neuronal death ; Fluoro-Jade ; Fluorescence labeling
• 刊名：Journal of Neuroscience Methods
• 出版年：2012
• 出版时间：30 June, 2012
• 年：2012
• 卷：208
• 期：1
• 页码：40-43
• 全文大小：2177 K

文摘

Neurodegeneration is the underlying cause of a vast majority of neurological disorders and often a result of brain trauma, stroke, or neurotoxic insult. Here we describe a simple method for labeling degenerating neurons in unfixed brain tissue samples. This method could provide a new avenue for identifying and harvesting degenerative neurons from unfixed brain tissues for subsequent molecular analyses.