TransgeneOmics - A transgenic platform for protein localization based function exploration
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- 作者：Susanne Hasse ; Anthony A. Hyman ; Mihail Sarov
- 关键词：BAC transgenesis ; Recombineering ; Protein localization ; Genome engineering ; Protein tagging
- 刊名：Methods
- 出版年：2016
- 出版时间：1 March 2016
- 年：2016
- 卷：96
- 期：Complete
- 页码：69-74
- 全文大小：847 K
文摘
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We review the use of gDNA transgenes for tag-based protein function exploration.
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We compare gDNA, cDNA, genome editing and antibody based approaches.
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We highlight the system-scale gDNA resources in worms, flies and mammalian cell.
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gDNA transgenics combines ease of use with near endogenous regulatory control.