- 作者：Noriko Ishizuka ; Akira Senoo ; Kaori Hayashi ; Kahoru Sasaki ; Masako Kako ; Yoko Suzuki ; Nobuo Imazeki ; Hiroyuki Shimizu ; Yoko Kobayashi ; Ryota Haba ; Tosei Takahashi ; Katsumi Arai ; Toshimasa Osaka ; Yuri Kintaka ; Yuichi Suzuki ; Shuji Inoue
- 关键词：Ventromedial hypothalamic lesions ; Mice ; Small intestine ; Proliferating cell nuclear antigen (PCNA)-positive cell ; Mitotic cell ; Cell proliferation
- 刊名：Obesity Research Clinical Practice
- 出版年：2012
- 出版时间：July-September, 2012
- 年：2012
- 卷：6
- 期：3
- 页码：e241-e247
- 全文大小：897 K
Summary
Background
We have found previously that ventromedial hypothalamic lesions (VMH) enhance cell proliferation in the visceral organs through vagal hyperactivity in rats. The goal of the current study was to determine the characteristics and nature of cell proliferation in the small intestine in VMH-lesioned mice.Methods
The weight and length of the small intestine, thickness of the mucosal and muscle layers, number of proliferating cell nuclear antigen (PCNA)-positive cells, and mitotic cell count in the mucosal layer in VMH-lesioned and Sham VMH-lesioned mice were determined at 7 days after the operation.
Results
The weight and length of the small intestine in VMH-lesioned mice were significantly greater than those in Sham VMH-lesioned mice, by 11.6 % and 15.0 % , respectively. The thicknesses of the mucosal and muscle layers of the small intestine in VMH-lesioned mice were also significantly greater than those in Sham VMH-lesioned mice, by 12.7 % and 12.5 % , respectively. PCNA-positive cells and mitotic cells in the mucosal layer were densely present in crypts in VMH-lesioned mice, and were significantly increased by 31.9 % and 71.7 % , respectively, compared to Sham VMH-lesioned mice.
Conclusions
These results demonstrate that VMH lesions in mice enhance cell proliferation in the mucosal layers and cause cell hypertrophy or cell proliferation in the muscle layers of the small intestine, which increases the weight and length of the small intestine. VMH lesions in mice may be a new tool for identifying growth factors and related genes involved in enlarging the small intestine mainly through cell proliferation.