- 作者：Noriko Ishizuka ; Nobuo Imazeki ; Akira Senoo ; Junko Sakurai ; Masaru Sonoda ; Masao Kanazawa ; Yoko Suzuki ; Yoko Kobayashi ; Tosei Takahashi ; Ryota Haba ; Katsumi Arai ; Hiroyuki Shimizu ; Kahoru Sasaki ; Masako Kako ; Kaori Hayashi ; Yuichi Suzuki ; Shuji Inoue
- 关键词：AGML ; Ventromedial hypothalamic lesions ; Rat ; Ulcer index ; Aggressive factor ; Defensive factor ; Proliferating cell nuclear antigen (PCNA)-labeling index ; Cell proliferation
- 刊名：Obesity Research Clinical Practice
- 出版年：2012
- 出版时间：July-September, 2012
- 年：2012
- 卷：6
- 期：3
- 页码：e233-e240
- 全文大小：891 K
Aim
The role of mucosal layer thickness on prevention of acute gastric mucosal lesions (AGMLs) was examined in ventromedial hypothalamic (VMH)-lesioned rats.Materials and methods
The incidence of AGMLs after 48-h fasting and 60 % ethanol injection into the stomach after 24-h fasting, aggressive factors (gastric acid and serum gastrin) and defensive factors [hexosamine, gastric mucosal blood flow (GMBF), serum thiobarbituric acid reacting substances (TBARS), and thickness of the gastric mucosal layer] were evaluated in VMH-lesioned rats. The effects of cell proliferation on the gastric mucosal layer of these rats were evaluated by H-E staining and immunostaining with proliferating cell nuclear antigen (PCNA).
Results
After 48-h fasting, no AGMLs were observed in VMH-lesioned and sham VMH-lesioned rats (controls). With 60 % ethanol administration after 24-h fasting, the numbers of AGMLs were similar in the two groups, but the ulcer index, a marker of ulcer formation, was lower in VMH-lesioned rats compared to that in sham VMH-lesioned rats. VMH-lesioned rats showed increased gastric acid secretion and serum gastrin compared to sham VMH-lesioned rats, indicating an increase in aggressive factors in VMH-lesioned rats. The two groups had similar levels of gastric mucosal hexosamine, GMBF, and gastric mucosal TBARS, but VMH-lesioned rats had an increased thickness of the mucosal cell layer, indicating an increase in defensive factors in these rats. Histologically, VMH-lesioned rats had an increased total mucosal cell layer, especially for the surface epithelial cell layer, and an increased PCNA-labeling index, a marker of cell proliferation, especially in the proliferative zones of gastric mucosa, indicating increased cell proliferation in the proliferative zone of the gastric mucosa.
Conclusion
VMH-lesioned rats are resistant to AGML formation due to increased cell proliferation in gastric mucosa through elevating the levels of defensive factors over those of aggressive factors.