Enhancement of solubility, purification and inclusion-bodies-refolding of an active pectin lyase from Penicillium occitanis expressed in Escherichia coli

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• 作者：Azza Hadj Sassi ; Hè ; la Trigui-Lahiani ; Salma Abdeljalil ; Ali Gargouri ; ^{Faouzi.gargouri@cbs.rnrt.tn}
• 关键词：Pectin lyase ; Heterologous expression ; Inclusion bodies
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：95
• 期：Complete
• 页码：256-262
• 全文大小：1187 K
• 卷排序：95

文摘

Analysis and structure modeling of pectin lyase protein sequence. Production optimization of a soluble and active recombinant pectin lyase. One step purification of the pectin lyase expressed in E. coli. Solubilisation and refolding of the pectin lyase from inclusion bodies.