Interactome of the inhibitory isoform of the nuclear transporter Importin 13

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• 作者：Shadma Fatima^a ; Kylie M. Wagstaff^a ; Kim G. Lieu^a ; Rebecca G. Davies^a ; Satomi S. Tanaka^b ; ^c ; Yasuka L. Yamaguchi^b ; ^c ; Kate L. Loveland^a ; ^d ; Patrick P.L. Tam^c ; ^e ; David A. Jans^a ; ^{david.jans@monash.edu}
• 关键词：Imp13 ; Importin 13 ; tImp13 ; testis specific N-terminal truncated isoform of full length Importin 13 ; NRNI ; negative regulator of nuclear import ; EIF4G2 ; eukaryotic translation initiation factor 4γ2 ; HMG20A ; high mobility group containing protein 20A ; GR ; glucocorticoid receptor ; NLS ; nuclear localisation signal ; ESCs ; embryonic stem cells ; CLSM ; confocal laser scanning microscopy ; Fn/c ; ratio of specific nuclear to cytoplasmic fluorescence above background
• 刊名：Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：1864
• 期：3
• 页码：546-561
• 全文大小：5097 K
• 卷排序：1864

文摘

A suite of interactors for the testis-specific form of Importin 13 were identified, and a number validated in mouse testis Translation initiation factor EIF4G2 and High Mobility Group 20A protein were identified as Importin 13 nuclear export cargo The testis-specific Importin 13 inhibited nuclear accumulation of all cargo examined, including glucocorticoid receptor Imp13 was detected for the first time in spermatozoa, along with specific binding partners, implying a role in mature sperm