- 作者：Satoshi Okamoto ; Hitoshi Niikura ; Kadzuki Nakabayashi ; Kayo Hiyama ; Maki Matoda ; Nobuhiro Takeshima ; Mika Watanabe ; Satoru Nagase ; Takeo Otsuki ; Nobuo Yaegashi
- 关键词：One-step nucleic acid amplification (OSNA) ; Cytokeratin 19 ; Cervical cancer ; Sentinel lymph node (SLN) ; Micrometastasis
- 刊名：Gynecologic Oncology
- 出版年：2013
- 出版时间：September, 2013
- 年：2013
- 卷：130
- 期：3
- 页码：530-536
- 全文大小：502 K
Objective
The purpose of this study was to examine the utility of the one-step nucleic acid amplification (OSNA) assay using cytokeratin (CK) 19 (KRT19) messenger RNA (mRNA) for the detection of sentinel lymph node (SLN) metastases in cervical cancer patients.Methods
To determine a cutoff value, KRT19 mRNA was assessed by OSNA assay using 239 lymph nodes (LNs) (217 histopathologically negative LNs and 22 positive LNs). A cutoff value was determined by statistical analysis of the copy numbers obtained by OSNA assay. Subsequently, performance evaluation of the OSNA assay (applying the cutoff value above) on 130 SLNs (32 patients) was used to investigate (through concordance) whether the OSNA assay exhibited diagnostic performance equivalent to the two-mm interval histopathological examination.
Results
Two hundred fifty copies/¦ÌL of KRT19 mRNA in the OSNA assay appeared to be an optimal cutoff value. In performance evaluation of the OSNA assay, we identified five positive SLNs and 125 negative SLNs by OSNA assay using KRT19 mRNA, exhibiting 96.2 % agreement with two-mm interval histopathological examination.
Conclusions
Our results indicated that the KRT19 mRNA OSNA assay can detect LN metastases as accurately as two-mm interval histopathological examination and thus may be an effective additional or alternative method for a rapid intra-operative examination of SLNs in cervical cancer.