A positive role of mammalian Tip41-like protein, TIPRL, in the amino-acid dependent mTORC1-signaling pathway through interaction with PP2A
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- 作者：Akio Nakashima ; Keiko Tanimura-Ito ; Noriko Oshiro ; Satoshi Eguchi ; Takafumi Miyamoto ; Ayaka Momonami ; Shinji Kamada ; Kazuyoshi Yonezawa ; Ushio Kikkawa
- 关键词：4E-BP1 ; eIF4E-binding protein 1 ; HA ; hemagglutinin ; HCC ; hepatocellular carcinoma ; PP2A ; protein phosphatase 2A ; PP2Ac ; PP2A catalytic subunit ; siRNA ; small interfering RNA ; S6K1 ; p70 ribosomal protein S6 kinase 1 ; Tap42 ; type 2A phosphatase-associated pr
- 刊名：FEBS Letters
- 出版年：2013
- 出版时间：17 September, 2013
- 年：2013
- 卷：587
- 期：18
- 页码：2924-2929
- 全文大小：894 K
文摘
Target of rapamycin complex 1 (TORC1) has a key role in cellular regulations in response to environmental conditions. In yeast, Tip41 downregulates TORC1 signaling via activation of PP2A phosphatase. We show here that overexpression of TIPRL, a mammalian Tip41, suppressed dephosphorylation of mechanistic TORC1 (mTORC1) substrates under amino acid withdrawal, and knockdown of TIPRL conversely attenuated phosphorylation of those substrates after amino acid refeeding. TIPRL associated with the catalytic subunit of PP2A (PP2Ac), which was required for the TIPRL action on mTORC1 signaling. Collectively, unlike yeast TIP41, TIPRL has a positive effect on mTORC1 signaling through the association with PP2Ac.