Streptomyces anulatus induced production of nitric oxide and cytotoxicity in human alveolar type 11 epithelial cells (A549)
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- 作者：Hirvonen ; M.-R. ; Jussila ; J. ; Ruotsalainen ; M. ; Savolainen ; K. ; Nevalainen ; A.
- 刊名：Toxicology Letters
- 出版年：1998
- 出版时间：July, 1998
- 年：1998
- 卷：95
- 期：Supplement 1
- 页码：112
- 全文大小：108 K
文摘
Dampness and mold growth in buildings cause spore generation into indoor air, which is associated with respiratory tract disorders. Specific agents or cellular mechanisms of diseases have not yet been identified. A likely contributing factor in the etiological mechanism of these respiratory symptoms could be inflammatory response towards specific organic materials in the microbes. NO plays an important role as an immune defense molecule. A variety of cells in the airways can be stimulated to produce large amounts of NO for long periods of time through stimulation of inducible NO synthase (iNOS). The ability of Streptomyces anulatus, gram positive bacteria which was isolated from moldy buildings, and lipopolysaccharide (LPS) or interferon gamma (IFN) to induce NO production and to cause cytotoxicity in human alveolar type 11 epithelial cells (A549) were studied. Here were report that Streptomyces anulatus or IFN induce a significant dose-, and time dependent increase in NO-production and decrease in the cell viability of A549 cells. The effects induced by Streptomyces anulatus alone, were further increased by IFN. LPS was not able to induce NO-production in these cells or to cause cytotoxicity. The present results suggests that Streptomyces anulatus, initiates a cascade of events in human alveolar A549 cells leading to cytotoxicity and production of NO, which is believed to be an important inflammatory mediator in the pathogenesis of many respiratory diseases.