Expression of IL-1 and IL-6 and their natural regulators in leukocytes of B-cell chronic lymphocytic leukaemia patients

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• 作者：Marzena Garley^a ; ^{marzena.garley@umb.edu.pl" class="auth_mail" title="E-mail the corresponding author} ; Ewa Jabłońska^a ; Jolanta Sawicka-Powierza^b ; Janusz Kłoczko^c ; Jarosław Piszcz^c ; Magdalena Kakareko^a ; Wioletta Ratajczak-Wrona^a ; Angelika Charkiewicz^d ; Wioleta Omeljaniuk^d ; Andrzej Szpak^d
• 关键词：B-CLL ; Interleukins ; B-lymphocytes ; Neutrophils
• 刊名：Advances in Medical Sciences
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：61
• 期：2
• 页码：187-192
• 全文大小：569 K

文摘

The purpose of the study was the assessment of the expression of IL-1β and IL-6, and the proteins regulating their biological activity, namely IL-1RII, IL-1Ra, as well as sIL-6Rα, sgp-130 in leukemic lymphocytes and autologous neutrophils of B-CLL patients.

Material/methods

The study involved a group of B-cell chronic lymphocytic leukemia patients and healthy volunteer blood donors. The presence of chosen proteins and their natural regulators was confirmed by Western blot.

Results

Western blot analysis showed a decreased expression of IL-1β and IL-6 in the leukocytes of B-CLL patients. Decreased expression of sIL-6Rα has been observed in lymphocytes, with a simultaneous increase of expression in PMNs. Lower expression of sgp-130 was found in B cells while its expression was elevated in the neutrophils of patients in early stages of the disease.

Conclusions

The changes observed in the expression of IL-1 and IL-6 seem to exclude their immediate involvement in the progress of B-CLL. However, the presented changes in the expression of proteins regulating IL-1β and IL-6 in PMNs indicate a potential role of early immune response cells also in advanced stages of the disease.