Dual sugar gut-permeability testing on blood drop in animal models
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- 作者：Katouzian ; Fourogh ; Sblattero ; Daniele ; Not ; Tarcisio ; Tommasini ; Alberto ; Giusto ; Elena ; Meiacco ; Daniela ; et. al.
- 关键词：Blood-drop ; Intestinal permeability ; Lactulose ; l-rhamnose ; Zonula occludens toxin-derived ????G molecule
- 刊名：Clinica Chimica Acta
- 出版年：2005
- 出版时间：February, 2005
- 年：2005
- 卷：352
- 期：1-2
- 页码：191-197
- 全文大小：224 K
文摘
Intestinal permeability is determined by measuring nonmetabolized sugars. In animals, intestinal permeability is determined in urine, using cumbersome and expensive metabolic cages. We developed an HPLC method for determining concentrations of lactulose (L) and l-rhamnose (R) in blood-drop of rabbits and mice, and we compared these results with the procedure based on sugars excreted in urine. We measured the intestinal permeability induced by a fragment (??G) of the zonula occludens toxin which opens the paracellular pathway.