Utilization of spent coffee grounds for isolation and stabilization of Paenibacillus chitinolyticus CKS1 cellulase by immobilization

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• 作者：Aneta V. Buntić ; ^{anetabuntic@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Marija D. Pavlović ; Du&scaron ; an G. Antonović ; Slavica S. &Scaron ; iler-Marinković ; Suzana I. Dimitrijević-Branković
• 关键词：Biotechnology ; Food science ; Materials science ; Bioengineering ; Ecology
• 刊名：Heliyon
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：2
• 期：8
• 全文大小：827 K

文摘

This study has explored the feasibility of using spent coffee grounds as a good supporting material for the Paenibacillus chitinolyticus CKS1 cellulase immobilization. An optimal operational conditions in a batch-adsorption system were found to be: carrier mass of 12 g/L, under the temperature of 45 °C and no pH adjustments. The immobilization yield reached about 71%. An equilibrium establishment between the cellulase and the carrier surface occurred within 45 min, whereas the process kinetics may be predicted by the pseudo-second-order model. An immobilized cellulase preparation expressed very good avicelase activity, this reached up to 2.67 U/g, and revealed an improved storage stability property, compared to free enzyme sample counterpart. The addition of metal ions, such as K⁺ and Mg²⁺ did not affect positively immobilization yield results, but on the contrary, contributed to an improved bio-activities of the immobilized cellulase, thus may be employed before each enzyme application. The method developed in this study offers a cheap and effective alternative for immediate enzyme isolation from the production medium and its stabilization, compared to other carriers used for the immobilization.