Screening and characterization of molecules that modulate the biological activity of IFNs-I
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- 作者：Milagros Bü ; rgia ; Viktor A. Zapol&rsquo ; skiib ; Bettina Hinkelmannc ; Mario Kö ; sterd ; Dieter E. Kaufmannb ; Florenz Sassec ; Hansjö ; rg Hauserd ; Marina Etcheverrigaraya ; Ricardo Kratjea ; Mariela Bollati-Fogolí ; ne ; mbollati@pasteur.edu.uy" class="auth_mail" title="E-mail the corresponding author ; Marcos Oggeroa
- 关键词：APA ; antiproliferative assay ; AVA ; antiviral assay ; C+ ; positive control ; C- ; negative control ; CC ; compound control ; CWT ; cells without treatment ; DMSO ; dimethyl sulfoxide ; EGFP ; enhanced green fluorescent protein ; EnE ; enhancer effect ; FCS ; fetal calf serum ; FSC ; foward scatter ; GepA ; Gephryronic acid A ; hIFNs-I ; human type I interferons ; HTS ; high throughput screening ; IFNs ; interferons ; IFNs-I ; type I interferons ; IFN ; + ; C ; co-incubation control ; IRF7 ; interferon response factor 7
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：10 September 2016
- 年：2016
- 卷：233
- 期：Complete
- 页码：6-16
- 全文大小：1927 K
文摘
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A low throughput assay to identify modulators of interferons activity is described.
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The assay was adapted to a range of cell-lines covering several cellular environments.
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Two collections of 154 natural and 288 synthetic compounds respectively were tested.
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One enhancer and 5 inhibitors of interferon activity were identified.