- 作者：Stefan Wagner ; Christian Radauer ; Merima Bublin ; Karin Hoffmann-Sommergruber ; Tamara Kopp ; Elli K. Greisenegger ; Lothar Vogel ; Stefan Vieths ; Otto Scheiner ; Heimo Breiteneder
- 关键词：Bet v 1 ; Bet v 1.0101 ; Bet v 1.0401 ; Bet v 1.1001 ; isoforms ; birch pollen allergy ; hypoallergenic variant
- 刊名：Journal of Allergy and Clinical Immunology
- 出版年：2008
- 出版时间：January 2008
- 年：2008
- 卷：121
- 期：1
- 页码：246-252
- 全文大小：854 K
Background
Engineered hypoallergens are currently being investigated for specific immunotherapy of allergic diseases in preclinical and clinical studies. Naturally occurring hypoallergens have by and large not been considered as a source of vaccine candidates.Objective
Evaluation of the antibody response in atopic individuals induced by birch pollen containing isoforms of the major birch pollen allergen Bet v 1.
Methods
Isoform-specific antibody isotype responses for Bet v 1.0101, Bet v 1.0401, and Bet v 1.1001 were determined for 35 sera of individuals with birch pollen allergy. Isoform structures were compared and related to IgE-binding inhibitory capacities and induction of mediator release in human Fc receptor transformed rat basophilic leukemia cells.
Results
Bet v 1.0101 induced a predominant IgE response, whereas the significant highest levels of IgG4 antibodies were directed against Bet v 1.0401. Bet v 1.1001 induced only a minimal antibody response. Structural comparisons revealed that most of the amino acid differences between the isoforms were located on the protein surfaces. IgE induced by Bet v 1.0101 only partly cross-reacted with the 2 other isoforms and bound to them with notably lower affinity. Bet v 1.0401 and Bet v 1.1001 also were poor inducers of mediator release.
Conclusion
Bet v 1 isoforms possess highly variant immunogenic and allergenic properties. Bet v 1.0101 acts as the sensitizing agent, whereas Bet v 1.0401 and Bet v 1.1001 can induce only a minimal IgE response.