Determination of aqueous two-phase system phase-forming components in the presence of bovine serum albumin

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• 作者：Anna Glyk ; Thomas Scheper ; Sascha Beutel ; ^{beutel@iftc.uni-hannover.de" class="auth_mail}
• 关键词：Aqueous two-phase system ; Refractive index measurement ; Conductivity measurement
• 刊名：Analytical Biochemistry
• 出版年：15 June 2014
• 年：2014
• 卷：455
• 期：Complete
• 页码：10-12
• 全文大小：486 K

文摘

In the current work, the quantification of different poly(ethylene glycol) (PEG)–potassium phosphate/sodium citrate aqueous two-phase system (ATPS) phase-forming components was investigated by using conductivity and refractive index measurements. For this purpose, refractive index and conductivity calibration curves were obtained for ATPS at different pH values in the presence of different bovine serum albumin (BSA) concentrations. Whereas BSA had no effect on the conductivity, it had a considerable effect on the refractive index. Finally, a convenient dilution of the samples prior to the ATPS constituent determination is needed to ensure no significant influence from BSA.