IL-4 -588C>T polymorphism and IL-4 receptor alpha [Ex5+14A>G; Ex11+828A>G] haplotype concur in selecting H. pylori cagA subtype infections

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• 作者：Carlo-Federico Zambon ; Daniela Basso ; Alberto Marchet ; Michela Fasolo ; Alessia Stranges ; Stefania Schiavon ; Filippo Navaglia ; Eliana Greco ; Paola Fogar ; Aless ; ra Falda ; Anna D'Odorico ; Massimo Rugge
• 关键词：IL-4 ; IL-4RA ; Gene polymorphism ; H. pylori ; Non-cardia gastric cancer
• 刊名：Clinica Chimica Acta
• 出版年：2008
• 出版时间：March 2008
• 年：2008
• 卷：389
• 期：1-2
• 页码：139-145
• 全文大小：263 K

文摘

The Th2 cytokine IL-4 might limit H. pylori associated gastric inflammation and favour H. pylori clearance. The aim of the study was to verify whether IL-4 -588C>T SNP, or two SNPs of the gene coding the alpha chain of IL-4 receptor (IL-4RA Ex5+14A>G, IL-4RA Ex11+828A>G) considered singly or as haplotypes, are correlated with H. pylori virulence genes or H. pylori associated diseases.

Methods

We studied 144 patients with non-cardia gastric cancer (NCGC)(41/50 with present or past H. pylori infection), 75 with duodenal ulcer (DU)(66 H. pylori infected) and 171 with gastritis (CG)(107 H. pylori infected). cagA gene was present in 24/28 NCGC, 45/59 DU and 56/107 CG.

Results

All SNPs were in Hardy–Weinberg equilibrium. IL-4RA haplotypes frequencies were estimated using Arlequin software. Neither the SNPs nor the IL-4RA haplotype correlated with disease diagnosis, H. pylori infection, degree of mucosal inflammation or intestinal metaplasia. IL-4 -588T allele (OR = 3.69, 95 % CI:1.34–10.16) and IL-4RA GA haplotype (p < 0.05) enhanced the risk for cagA positive infections. IL-4RA GA haplotype correlated with IL-4 protein levels in H. pylori infected gastric mucosa.

Conclusions

IL-4 and IL-4RA gene polymorphisms concur in selecting the H. pylori infecting strain, probably influencing the IL-4 signalling pathway.