Adherence of human mesenchymal stem cells on Ti and TiO₂ nano-columnar surfaces fabricated by glancing angle sputter deposition

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• 作者：Yahya Motemani ; Christina Greulich ; Chinmay Khare ; Michael Lopian ; Pio John S. Buenconsejo ; Thomas A. Schildhauer ; Alfred Ludwig ; Manfred K枚ller
• 关键词：Human mesenchymal stem cells ; Cell adhesion ; Nanostructure ; Titanium ; Glancing angle sputter deposition
• 刊名：Applied Surface Science
• 出版年：15 February, 2014
• 年：2014
• 卷：292
• 期：Complete
• 页码：626-631
• 全文大小：2328 K

文摘

The interaction of human mesenchymal stem cells (hMSCs) with Ti and TiO₂ nano-columnar surfaces fabricated using glancing angle sputter deposition was investigated. The adherence and proliferation of hMSCs on different nano-columnar surfaces, including vertical columns, slanted columns and chevrons, were examined with calcein-acetoxymethyl ester fluorescence staining and scanning electron microscopy. For comparison, adherence of hMSCs on compact, dense films was also studied. After 24 h and 7 days, adherent and viable cells were observed on both, Ti nano-columns as well as dense Ti films, which confirms the biocompatibility of these nanostructures. Very small pseudopodia with width of approximately 20-35 nm and length varying from 20 to 200 nm were observed between the nano-columns, independent of the type of the nano-columnar morphology. Large inter-column spacing and effectively increased surface area make these nanostructures promising candidates for bio-functionalization or drug loading on the surface of Ti-based implants.