Cross-reactivity of anti-PLA2R1 autoantibodies to rabbit and mouse PLA2R1 antigens and development of two novel ELISAs with different diagnostic performances in idiopathic membranous nephropathy
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- 作者：Barbara Seitz-Polskia ; b ; c ; seitz-polski.b@chu-nice.fr" class="auth_mail" title="E-mail the corresponding author ; Guillaume Dollaa ; 1 ; Christine Payré ; a ; 1 ; Nicola M. Tomasa ; d ; Marine Lochouarna ; b ; c ; Louise Jeammeta ; Christophe Mariate ; Thierry Krummelf ; Sté ; phane Burteyg ; Cé ; cile Courivaudh ; Wolfgang Schlumbergeri ; Ké ; vin Zorzij ; Sylvia Benzakenc ; Ghislaine Bernardc ; Vincent L.M. Esnaultb ; 2 ; Gé ; rard Lambeaua ; 2 ; lambeau@ipmc.cnrs.fr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Autoimmunity ; Conformational epitope ; ELISA ; Membranous nephropathy ; PLA2R1 autoantibodies ; Mouse PLA2R1 ; Rabbit PLA2R1 ; Orthologs
- 刊名：Biochimie
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：118
- 期：Complete
- 页码：104-115
- 全文大小：1391 K
文摘
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PLA2R1 is the major autoantigen in membranous nephropathy, a human kidney disease.
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Only human PLA2R1 has been used as antigen to measure anti-PLA2R1 autoantibodies.
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We found rabbit PLA2R1 as an alternative antigen to human PLA2R1.
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We found mouse PLA2R1 as a specific antigen detecting a subset of autoantibodies.
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Rabbit and mouse ELISAs have different diagnosis and prognosis performances.