A Kunitz type protease inhibitor related protein is synthesized in Drosophila prepupal salivary glands and released into the moulting fluid during pupation

详细信息    查看全文

• 作者：Kress ; Horst ; Jarrin ; Andres ; Thü ; roff ; Eduardo ; Saunders ; Robert ; Weise ; Chris ; Schmidt am Busch ; Marcel ; et. al.
• 关键词：Drosophila ; Prepupal salivary gland ; Kunitz serine protease inhibitor ; Ecdysone ; Metamorphosis ; Pupation fluid
• 刊名：Insect Biochemistry and Molecular Biology
• 出版年：2004
• 出版时间：August, 2004
• 年：2004
• 卷：34
• 期：8
• 页码：855-869
• 全文大小：1021 K

文摘

From the Drosophila virilis late puff region 31C, we microcloned two neighbouring genes, Kil-1 and Kil-2, that encode putative Kunitz serine protease inhibitor like proteins. The Kil-1 gene is expressed exclusively in prepupal salivary glands. Using a size mutant of the KIL-1 protein and MALDI-TOF analysis, we demonstrate that during pupation this protein is released from the prepupal salivary glands into the pupation fluid covering the surface of the pupa. 3-D-structure predictions are consistent with the known crystal structure of the human Kunitz type protease inhibitor 2KNT. This is the first experimental proof for the extracorporal presence of a distinct Drosophila prepupal salivary gland protein. Possible functions of KIL-1 in the context of the control of proteolytic activities in the pupation fluid are discussed.