Production and characterization of iron sulfur cluster dihydroxyacid dehydratase.
SsDHAD catalyzes the non-natural dehydration of d-glycerate to pyruvate.
Specific activity of SsDHAD could be increased by 2-mercaptoethanol up to 3-fold.
Storage and stability parameters of SsDHAD were analyzed and compared with regard to biofuel production.
SsDHAD can be used for a convenient one-step synthesis route from d-gluconate to KDG.