Characterization of recombinantly expressed dihydroxy-acid dehydratase from Sulfobus solfataricus鈥擜 key enzyme for the conversion of carbohydrates into chemicals
详细信息 查看全文
- 作者：Jö ; rg M. Carsten Joerg.carsten@tum.de" class="auth_mail" title="E-mail the corresponding author ; Anja Schmidt Anja.philipp@tum.de" class="auth_mail" title="E-mail the corresponding author ; Volker Sieber ; Sieber@tum.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：Dihydroxyacid dehydratase ; Iron sulfur cluster ; 2-Keto-3-deoxy-gluconate ; d-Glycerate ; Synthetic cascade biomanufacturing
- 刊名：Journal of Biotechnology
- 出版年：2015
- 出版时间：10 October 2015
- 年：2015
- 卷：211
- 期：Complete
- 页码：31-41
- 全文大小：1120 K
文摘
- •
Production and characterization of iron sulfur cluster dihydroxyacid dehydratase.
- •
SsDHAD catalyzes the non-natural dehydration of d-glycerate to pyruvate.
- •
Specific activity of SsDHAD could be increased by 2-mercaptoethanol up to 3-fold.
- •
Storage and stability parameters of SsDHAD were analyzed and compared with regard to biofuel production.
- •
SsDHAD can be used for a convenient one-step synthesis route from d-gluconate to KDG.