- 作者：Ozan Y¨¹ksel Tekta? ; Ajay Yadav ; Fabian Garreis ; Ursula Schl?tzer-Schrehardt ; Martin Schicht ; Ulrike Hampel ; Lars Br?uer ; Friedrich Paulsen
- 关键词：Dry eye syndrome ; Meibomian gland dysfunction ; Eyelid ; Mucocutaneous junction ; Cytokeratins ; Mucins ; Conjunctival stem cells
- 刊名：Annals of Anatomy - Anatomischer Anzeiger
- 出版年：2012
- 出版时间：September, 2012
- 年：2012
- 卷：194
- 期：5
- 页码：436-445
- 全文大小：2743 K
Summary
Purpose
To investigate the morphology of the human eyelid margin and the presence of different cytokeratins, mucins and stem cell markers within the skin epithelium, mucocutaneous junction (MCJ) and palpebral conjunctiva.Methods
Eyelids of body donors were investigated histologically and ultrastructurally as well as by immunohistochemical methods using antibodies to cytokeratins 1, 4, 7, 8, 10, 13, 14, 15, and 19; mucins MUC1, MUC4, and MUC5AC and potential stem cell markers K15, BCRP/ABCG2, integrin ¦Â1, and N-cadherin.
Results
The expression pattern of cytokeratins, mucins and stem cell markers varied across the different epithelia of the human eyelid. Within the MCJ, CK7, 15 and 19 were absent, whereas the epithelium reacted positive to antibodies to CK1, 4, 8, 10, 13 and 14. Reactivity was also observed for MUC1 and MUC4, but not for MUC5AC. No reactivity was determined for K15, BCRP/ABCG2 and integrin ¦Â1 in the area of the MCJ epithelium but a strong reactivity was present for N-cadherin.
Conclusions
The present immunohistochemical findings lead to a better characterization of the MCJ. Additionally, the knowledge of distribution of biomarkers like cytokeratins, mucins and stem cells can be useful in the investigation of MCJ disturbances which occur in several disorders of the meibomian glands and the lid epithelium in the course of dry eye syndrome and especially meibomian gland dysfunction.