MHC Class I Gene Organization in >1.5-Mb YAC Contigs from the H2-M Region

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• 作者：Jones ; Elsy P. ; Xiao ; Hong ; Schultz ; Roger A. ; Flaherty ; Lorraine ; Trachtulec ; Zdenek ; Vincek ; Vladimir ; et. al.
• 刊名：Genomics
• 出版年：1995
• 出版时间：May 1, 1995
• 年：1995
• 卷：27
• 期：1
• 页码：40-51
• 全文大小：901 K

文摘

Sixteen yeast artificial chromosome (YAC) clones have been mapped to the H2-M region at the distal end of the mouse major histocompatibility complex (MHC) on chromosome 17. Analysis of the YACs with single-and multicopy probes yielded a proximal contig spanning a minimum of 800 kb and a distal contig of 700 kb. A probe for the conserved fourth exon of MHC class I genes detected 19 restriction fragments, including 6 of the 8 previously characterized H2-M class I genes, in the proximal contig. This contig spans the gap from the M to the T region and includes the T1 gene. By contrast, only two class I genes, M2 and M3, were found in the distal contig. These two genes, which are both expressed, may mark the end of the MHC. The order among nine class I genes and seven other markers was determined in the cloned DNA from the centromere as T1, Tu32A, (M1-M7-M8), Tu32B, B30, M6, M4, M5, Mog, Tu42A//M2, Leh525, M3, Tu42B, where the orientation with respect to the centromere is unknown for M1-M7-M8 .