Highly sensitive isotope-dilution liquid-chromatography-electrospray ionization-tandem-mass spectrometry approach to study the drug-mediated modulation of dopamine and serotonin levels in Caenorhabditis elegans
详细信息 查看全文
- 作者：Fabian Schumachera ; b ; Sudipta Chakrabortyc ; d ; Burkhard Kleusera ; Erich Gulbinsb ; Tanja Schwerdtlee ; Michael Aschnerd ; Julia Bornhorste ; julia.bornhorst@uni-potsdam.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：Caenorhabditis elegans ; Dopamine ; Serotonin ; Liquid chromatography-tandem mass spectrometry ; Isotope-dilution analysis
- 刊名：Talanta
- 出版年：2015
- 出版时间：1 November 2015
- 年：2015
- 卷：144
- 期：Complete
- 页码：71-79
- 全文大小：1292 K
文摘
- •
Highly sensitive LC-MS/MS method for DA and SRT quantification in C. elegans.
- •
Applied sample preparation protocol assures high recovery rates of DA and SRT.
- •
Exposure of C. elegans to MAO-B or COMT inhibitors result in an accumulation of DA.
- •
C. elegans well-suited to study drug-mediated modulation of the DA and SRT system.
- •
Behavioral readout of the treated worms corroborated the analytical LC-MS/MS data.