Toxicological characterisation of a thio-arsenosugar-glycerol in human cells

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• 作者：Franziska Ebert^a ; Sö ; ren Meyer^a ; ^b ; Larissa Leffers^a ; ^b ; Georg Raber^c ; Kevin A. Francesconi^c ; Tanja Schwerdtle^a ; ^{taschwer@uni-potsdam.de" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Arsenic ; Thio-arsenosugar-glycerol ; Toxicity ; Toxicokinetics ; Genotoxicity ; Metabolism
• 刊名：Journal of Trace Elements in Medicine and Biology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：38
• 期：Complete
• 页码：150-156
• 全文大小：1274 K

文摘

Arsenosugars are water-soluble arsenic species predominant in marine algae and other seafood including mussels and oysters. They typically occur at levels ranging from 2 to 50 mg arsenic/kg dry weight. Most of the arsenosugars contain arsenic as a dimethylarsinoyl group (Me₂As(O)-), commonly referred to as the oxo forms, but thio analogues have also been identified in marine organisms and as metabolic products of oxo-arsenosugars. So far, no data regarding toxicity and toxicokinetics of thio-arsenosugars are available. This in vitro-based study indicates that thio-dimethylarsenosugar-glycerol exerts neither pronounced cytotoxicity nor genotoxicity even though this arsenical was bioavailable to human hepatic (HepG2) and urothelial (UROtsa) cells. Experiments with the Caco-2 intestinal barrier model mimicking human absorption indicate for the thio-arsenosugar-glycerol higher intestinal bioavailability as compared to the oxo-arsenosugars. Nevertheless, absorption estimates were much lower in comparison to other arsenicals including arsenite and arsenic-containing hydrocarbons. Arsenic speciation in cell lysates revealed that HepG2 cells are able to metabolise the thio-arsenosugar-glycerol to some extent to dimethylarsinic acid (DMA). These first in vitro data cannot fully exclude risks to human health related to the presence of thio-arsenosugars in food.