Combination of the isolation using 96-well microplates and traditional UV mutagenesis for screening HCT mutant.
Microalgal mutant Chlorella vulgaris SDEC-3M was screened out by modified UV mutagenesis.
SDEC-3M showed high CO2 tolerance, high CO2 requiring and relevant genetic stability.
LCE and carbohydrate content of SDEC-3M were significantly elevated.
SDEC-3M offers a strong candidature as CO2 biofixation and biofuel production.