Chrysoporthe cubensis: A new source of cellulases and hemicellulases to application in biomass saccharification processes

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• 作者：Daniel Luciano Falkoski^a ; Valé ; ria Monteze Guimar&#227 ; es^a ; Maí ; ra Nicolau de Almeida^a ; Acelino Couto Alfenas^b ; Jorge Luiz Colodette^c ; Sebasti&#227 ; o Tavares de Rezende^a ; ^{srezende@ufv.br}
• 关键词：Chrysoporthe cubensis ; Plant pathogen ; Cellulases ; Hemicellulases ; Biomass saccharification
• 刊名：Bioresource Technology
• 出版年：2013
• 出版时间：February, 2013
• 年：2013
• 卷：130
• 期：Complete
• 页码：296-305
• 全文大小：798 K

文摘

The plant pathogenic fungus Chrysoporthe cubensis was cultivated under solid state employing different substrates and the highest endoglucanase (33.84 U g^?1), FPase (2.52 U g^?1), ¦Â-glucosidase (21.55 U g^?1) and xylanase (362.38 U g^?1) activities were obtained using wheat bran as carbon source. Cellulases and xylanase produced by C. cubensis showed maximal hydrolysis rate at pH 4.0 and in a temperature range of 50-60 ¡ãC. All enzymatic activities were highly stable at 40 and 50 ¡ãC through 48 h of pre-incubation. Saccharification of alkaline pretreated sugarcane bagasse by crude enzyme extract from C. cubensis resulted in release of 320.8 mg/g and 288.7 mg/g of glucose and xylose, respectively. On another hand, a similar assay employing commercial cellulase preparation resulted in release of 250.6 mg/g and 62.1 mg/g of glucose and xylose, respectively. Cellulolytic extract from C. cubensis showed a great potential to be used in biomass saccharification processes.