An antigen-specific, four-color, B-cell FluoroSpot assay utilizing tagged antigens for detection
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- 作者：Peter Jahnmatza ; b ; peter.jahnmatz@ki.se" class="auth_mail" title="E-mail the corresponding author ; Theresa Bengtssonb ; Bartek Zuberc ; Anna Fä ; rnerta ; e ; Niklas Ahlborgb ; d
- 关键词：ASC ; antibody-secreting cell ; BSA ; bovine serum albumin ; DENV ; dengue virus ; DMEM ; Dulbecco's modified Eagle's medium ; FCS ; fetal calf serum ; FITC ; Fluorescein isothiocyanate ; Ig ; immunoglobulin ; IFN-γ ; interferon-gamma ; IL ; interleukin ; KLH ; keyhole limpet hemocyanin ; mAb ; monoclonal antibody ; PBS ; phosphate-buffered saline ; RT ; room temperature ; SA ; streptavidin
- 刊名：Journal of Immunological Methods
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：433
- 期：Complete
- 页码：23-30
- 全文大小：1254 K
文摘
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A B-cell FluoroSpot system utilizing tag/anti-tag detection was developed.
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Antibody-secreting cells (ASCs) specific for different antigens could be detected.
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Antibody cross-reactivity could be assessed at the single-cell level.
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Antigen-specificity and Ig isotype/subclass could be determined from a single ASC.
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The assay could be adapted to studies of a variety of protein antigens.