Glassy carbon electrodes sequentially modified by cysteamine-capped gold nanoparticles and poly(amidoamine) dendrimers generation 4.5 for detecting uric acid in human serum without ascorbic acid interference

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• 作者：A.S. Ram铆rez-Segovia ; J.A. B ; a-Alem谩n ; S. Guti茅rrez-Granados ; A. Rodr铆guez ; F.J. Rodr铆guez ; Luis A. God铆nez ; E. Bustos ; J. Manr铆quez
• 关键词：Modified electrodes ; Human serum ; Uric acid ; Gold nanoparticles ; Dendrimers
• 刊名：Analytica Chimica Acta
• 出版年：17 February, 2014
• 年：2014
• 卷：812
• 期：Complete
• 页码：18-25
• 全文大小：5107 K

文摘

Glassy carbon electrodes (GCE) were sequentially modified by cysteamine-capped gold nanoparticles (AuNp@cysteamine) and PAMAM dendrimers generation 4.5 bearing 128-COOH peripheral groups (GCE/AuNp@cysteamine/PAMAM), in order to explore their capabilities as electrochemical detectors of uric acid (UA) in human serum samples at pH 2. The results showed that concentrations of UA detected by cyclic voltammetry with GCE/AuNp@cysteamine/PAMAM were comparable (deviation <卤10%; limits of detection (LOD) and quantification (LOQ) were 1.7 脳 10^鈭? and 5.8 脳 10^鈭? mg dL^鈭?, respectively) to those concentrations obtained using the uricase-based enzymatic-colorimetric method. It was also observed that the presence of dendrimers in the GCE/AuNp@cysteamine/PAMAM system minimizes ascorbic acid (AA) interference during UA oxidation, thus improving the electrocatalytic activity of the gold nanoparticles.