A novel ultra-sensitive enzyme immunoassay for soluble human insulin receptor ectodomain and its measurement in urine from healthy subjects and patients with diabetes mellitus

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• 作者：Asako Umehara ; Mamiko Nishioka ; Toshiyuki Obata ; Yousuke Ebina ; Hiroshi Shiota ; Seiichi Hashida
• 关键词：Enzyme immunoassay ; Insulin receptor ; Urine ; Diabetes ; Insulin ; Resistin ; Leptin
• 刊名：Clinical Biochemistry
• 出版年：2009
• 出版时间：September 2009
• 年：2009
• 卷：42
• 期：13-14
• 页码：1468-1475
• 全文大小：564 K

文摘

Objective

For the early identification of patients at risk of developing diabetes mellitus, and to prevent the onset of diabetes by performing dietary counseling and exercise guidance, we have developed an ultra-sensitive immune complex transfer enzyme immunoassay (ICT-EIA) to measure soluble human insulin receptor ectodomain (sIRα) in urine which is collected non-invasively.

Design and methods

We developed ICT-EIA for sIRα and measured urinary sIRα from 106 healthy volunteers, 35 obese volunteers and 42 patients with diabetes.

Results

The detection limit of ICT-EIA (0.04 pg/mL), using a urine sample of as little as 100 μL, was a few hundred-fold higher than that of conventional ELISA. Using ICT-EIA, the urinary sIRα level in patients with diabetes (9.7 ± 20.1 pg/mg creatinine) was significantly higher than those in healthy volunteers (1.4 ± 0.9; P < 0.001).

Conclusion

ICT-EIA for sIRα may be useful as a good marker for evaluating diabetes risk.