Inhibition of bile canalicular network formation in rat sandwich cultured hepatocytes by drugs associated with risk of severe liver injury
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- 作者：Akinori Takemura1 ; Aya Izaki1 ; Shuichi Sekine ; Kousei Ito ; itokousei@chiba-u.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：DILI ; drug-induced liver injury ; ALT ; alanine aminotransferase ; ALP ; alkaline phosphatase ; BBW ; black box warning ; WDN ; withdrawn ; BC ; bile canalicular ; BSEP ; bile salt export pump ; MRP2 ; multidrug resistance-associated protein 2 ; SCHs ; sandwich cultured hepatocytes ; WME ; Williams' Medium E ; DMEM ; Dulbecco's Modified Eagle's Medium ; TCA ; taurocholic acid ; LCA ; lithocholic acid ; LDH ; lactate dehydrogenase ; IC50 ; half maximal inhibitory concentration ; FDA ; Food and Drug Administration ; MDD ; maximu
- 刊名：Toxicology in Vitro
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：35
- 期：Complete
- 页码：121-130
- 全文大小：1597 K
文摘
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The bile canalicular network is a prerequisite for biliary excretion.
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Bile canalicular network formation was quantified in vitro.
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Some drugs potently inhibited bile canalicular network formation.
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Inhibition by drugs correlated with the severity of in vivo liver toxicity.
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Our novel assay system is useful to predict the risk of drug-induced liver injury.